Mouse Anti-Human GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35

• Catalog number
  SMC-346D-HRP
• Price
  Please ask
• Size
  100 µg

• Clone
  S95-35
• Immunogen
  Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1
• Antibody s full description
  Mouse Anti-Human GABA A Receptor Monoclonal IgG2A Antibody, Clone: S95-35: HRP
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  ECA4 antibody, EJM antibody, EJM5 antibody, Gaba receptor alpha 1 polypeptide antibody, GABA(A) receptor antibody, GABA(A) receptor subunit alpha 1 antibody, GABA(A) receptor subunit alpha-1 antibody, GABA(A) receptor, alpha 1 antibody, GABRA 1 antibody, GABRA1 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody, Gamma aminobutyric acid A receptor alpha 1 antibody, Gamma aminobutyric acid receptor subunit alpha 1 antibody, Gamma-aminobutyric acid receptor subunit alpha-1 antibody, GBRA1_HUMAN antibody
• Raised in
  Mouse
• Antibody s target
  GABA A Receptor
• Primary research fields
  Neuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF, AM
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or –Alpha3.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-346 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
• Bibliography
  1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
• Released date
  13-Apr-2010
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_034380.1
• Gene number
  14394
• Protein number
  P14867
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 (SMC-346). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells. Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 (SMC-346). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-GABA A Receptor Antibody [S95-35] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-346) Mouse Anti-GABA A Receptor Antibody [S95-35] used in Western Blot (WB) on Human Cell line lysates (SMC-346)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  The Mouse Anti- GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Latin name
  Mus musculus

• Gene target
  GABA-A   Receptor   Alpha-1   Monoclonal   IgG2A   S95-35  
• Gene symbol
  IGLV7-35, SNORD115-35, IGKV1-35, IGHV3-35, IGKV1D-35, ABAT, RNU6-35P, HLA-A, IGHV5-10-1, CIDEA
• Short name
  Mouse Anti- GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35
• Technique
  anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2A, IgG2a
• Label
  HRP
• Species
  Human, Humans, Mouses
• Alternative name
  Mouse Antibody toHuman GABA-A Receptor a-1 monoclonal IgG2A, clonality S95-35
• Alternative technique
  murine, antibodies
• Clone name
  S95-35

Gene info

• Identity
  HGNC:5928
• Gene
  IGLV7-35
• Long gene name
  immunoglobulin lambda variable 7-35 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 7-35
  • immunoglobulin lambda variable 7-35 pseudogene
• GenBank acession
  • Z73660
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28777
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151067

Gene info

• Identity
  HGNC:33054
• Gene
  SNORD115-35
• Long gene name
  small nucleolar RNA, C/D box 115-35
• Synonyms
  • HBII-52-35
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033809
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003350.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5738
• Gene
  IGKV1-35
• Long gene name
  immunoglobulin kappa variable 1-35 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1-35
  • immunoglobulin kappa variable 1-35 pseudogene
• GenBank acession
  • X71890
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28932
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151681

Gene info

• Identity
  HGNC:5598
• Gene
  IGHV3-35
• Long gene name
  immunoglobulin heavy variable 3-35 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 3-35
• GenBank acession
  • M99666
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28432
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152079

Gene info

• Identity
  HGNC:5754
• Gene
  IGKV1D-35
• Long gene name
  immunoglobulin kappa variable 1D-35 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1D-35
  • immunoglobulin kappa variable 1D-35 pseudogene
• GenBank acession
  • X71894
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28895
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151603

Gene info

• Identity
  HGNC:23
• Gene
  ABAT
• Long gene name
  4-aminobutyrate aminotransferase
• Synonyms
  • GABAT
  • GABA-T
• Synonyms name
  • 4-aminobutyrate transaminase
  • gamma-aminobutyrate aminotransferase
  • GABA transaminase
• GenBank acession
  • L32961
• Locus
  16p13.2
• Discovery year
  1996-03-13
• Entrez gene record
  18
• Pubmed identfication
  • 7721088
• RefSeq identity
  • NM_020686
• VEGA ID
  OTTHUMG00000048201

Gene info

• Identity
  HGNC:34279
• Gene
  RNU6-35P
• Long gene name
  RNA, U6 small nuclear 35, pseudogene
• Synonyms gene
  • RNU6-35
• Synonyms gene name
  • RNA, U6 small nuclear 35
• Locus
  4q25
• Discovery year
  2008-06-10
• Entrez gene record
  100873750
• RefSeq identity
  • NR_046493

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

Gene info

• Identity
  HGNC:1976
• Gene
  CIDEA
• Long gene name
  cell death inducing DFFA like effector a
• Synonyms gene name
  • cell death-inducing DFFA-like effector a
• Synonyms
  • CIDE-A
• GenBank acession
  • AF041378
• Locus
  18p11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1149
• Pubmed identfication
  • 9564035
  • 18509062
• RefSeq identity
  • NM_001279
• VEGA ID
  OTTHUMG00000131691

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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