Mouse Anti-Human GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35
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Catalog numberSMC-346D
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PricePlease ask
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Size100 µg
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CloneS95-35
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ImmunogenFusion protein amino acids 355-394 of human GABA-A-R-Alpha1
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Antibody s full descriptionMouse Anti-Human GABA A Receptor Monoclonal IgG2A Antibody, Clone: S95-35: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameECA4 antibody, EJM antibody, EJM5 antibody, Gaba receptor alpha 1 polypeptide antibody, GABA(A) receptor antibody, GABA(A) receptor subunit alpha 1 antibody, GABA(A) receptor subunit alpha-1 antibody, GABA(A) receptor, alpha 1 antibody, GABRA 1 antibody, GABRA1 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody, Gamma aminobutyric acid A receptor alpha 1 antibody, Gamma aminobutyric acid receptor subunit alpha 1 antibody, Gamma-aminobutyric acid receptor subunit alpha-1 antibody, GBRA1_HUMAN antibody
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Raised inMouse
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Antibody s targetGABA A Receptor
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Primary research fieldsNeuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or –Alpha3.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-346 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
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Bibliography1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_034380.1
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Gene number14394
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Protein numberP14867
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 (SMC-346). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells. Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 (SMC-346). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-GABA A Receptor Antibody [S95-35] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-346) Mouse Anti-GABA A Receptor Antibody [S95-35] used in Western Blot (WB) on Human Cell line lysates (SMC-346)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThe Mouse Anti- GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolIGLV7-35, SNORD115-35, IGKV1-35, IGHV3-35, IGKV1D-35, ABAT, RNU6-35P, HLA-A, IGHV5-10-1, CIDEA
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Short nameMouse Anti- GABA-A Receptor Alpha-1 Monoclonal IgG2A, Clone S95-35
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2A, IgG2a
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman GABA-A Receptor a-1 monoclonal IgG2A, clonality S95-35
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Alternative techniquemurine, antibodies
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Clone nameS95-35
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 7-35 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 7-35
- immunoglobulin lambda variable 7-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-35
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-35 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-35
- immunoglobulin kappa variable 1-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-35 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3-35
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-35 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-35
- immunoglobulin kappa variable 1D-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name4-aminobutyrate aminotransferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-03-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 35, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 35
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data