Mouse Anti-Human Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80
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Catalog numberSMC-435D-FITC
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PricePlease ask
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Size100 µg
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CloneS330A-80
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ImmunogenSynthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
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Antibody s full descriptionMouse Anti-Human Alpha2C Adrenergic Monoclonal IgG1 Antibody, Clone: S330A-80: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameAdra 2c Antibody, Alpha-2CAR Antibody, ADRA2C Antibody, ADRA2L2 Antibody, ADRARL2 Antibody, Alpha 2CAR Antibody, alpha2 AR C4 Antibody, alpha2 C4 Antibody, alpha2C Antibody, ALPHA2CAR Antibody, Alpha-2 adrenergic receptor subtype C4 Antibody, Alpha-2C adrenoreceptor Antibody, Adrenergic alpha 2C receptor Antibody, Alpha 2C adrenergic receptor Antibody, Alpha 2C adrenergic receptor subtype C4 Antibody, Alpha 2C adrenoceptor Antibody, Alpha 2C adrenoreceptor Antibody, Alpha-2-adrenergic receptor Antibody, renal type Antibody, alpha2cii-adrenergic receptor Antibody, Subtype alpha2 C4 Antibody
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Raised inMouse
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Antibody s targetAlpha2C Adrenergic
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Primary research fieldsNeuroscience, Neurotransmitter Receptors, Alpha-2 Adrenergic Receptors, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-435 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
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Bibliography1. Filipeanu C.M., et al. (2011) Biochim Biophys Acta. 1813: 346-357. 2. Powe D.G., et al. (2011) Breast Cancer Res Treat. 130: 457-463.
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Released date5-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_000674.2
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Gene number152
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Protein numberP18825
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (SMC-435). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (SMC-435) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (SMC-435). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (SMC-435) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-Alpha-2C Adrenergic Receptor Antibody [S330A-80] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-435) Mouse Anti-Alpha-2C Adrenergic Receptor Antibody [S330A-80] used in Western Blot (WB) on Monkey COS cells transfected with HA-tagged Alpha-2C (SMC-435)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThe Mouse Anti- Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV4-80, HLA-W, RNU6-80P, VNN2, UNC80, AASDHPPT, OR4H6P
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Short nameMouse Anti- Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman a 2C Adrenergic Receptor monoclonal IgG1, clonality S330A-80
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Alternative techniquemurine, antibodies
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Clone nameS330A-80
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-80 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 4-80
- immunoglobulin heavy variable 4-80 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, W (pseudogene)
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Synonyms gene
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Synonyms gene name
- HLA-80 pseudogene
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GenBank acession
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Locus
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Discovery year2004-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 80, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 80
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Locus
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Discovery year2011-08-08
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namevanin 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-13
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Entrez gene record
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Pubmed identfication
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Classification
- Vanin family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameunc-80 homolog, NALCN channel complex subunit
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Synonyms gene
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Synonyms gene name
- chromosome 2 open reading frame 21
- unc-80 homolog (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-12-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 4 subfamily H member 6 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 4, subfamily H, member 8 pseudogene
- olfactory receptor, family 4, subfamily H, member 6 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-25
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 4
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data