Mouse Anti-Human Alpha 2C Adrenergic Monoclonal IgG2A, Clone S330A-51
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Catalog numberSMC-433D-A488
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PricePlease ask
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Size100 µg
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CloneS330A-51
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ImmunogenSynthetic peptide amino acids 442-462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
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Antibody s full descriptionMouse Anti-Human Alpha-2C Adrenergic Monoclonal IgG2A Antibody, Clone: S330A-51: ATTO 488
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameAdra 2c Antibody, Alpha-2CAR Antibody, ADRA2C Antibody, ADRA2L2 Antibody, ADRARL2 Antibody, Alpha 2CAR Antibody, alpha2 AR C4 Antibody, alpha2 C4 Antibody, alpha2C Antibody, ALPHA2CAR Antibody, Alpha-2 adrenergic receptor subtype C4 Antibody, Alpha-2C adrenoreceptor Antibody, Adrenergic alpha 2C receptor Antibody, Alpha 2C adrenergic receptor Antibody, Alpha 2C adrenergic receptor subtype C4 Antibody, Alpha 2C adrenoceptor Antibody, Alpha 2C adrenoreceptor Antibody, Alpha-2-adrenergic receptor Antibody, renal type Antibody, alpha2cii-adrenergic receptor Antibody, Subtype alpha2 C4 Antibody
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Raised inMouse
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Antibody s targetAlpha-2C Adrenergic
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Primary research fieldsNeuroscience, Neurotransmitter Receptors, Alpha-2 Adrenergic Receptors, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis3 µg/ml of SMC-433 was sufficient for detection of Alpha-2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha-2Cmembrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secon_x0000__x0000__x0000__x0000__x0000__x0000_body._x0000_y.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
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Bibliography1. Filipeanu C.M., et al. (2011) Biochim BiophysActa. 1813: 346-357. 2. Powe D.G., et al. (2011) Breast Cancer Res Treat. 130: 457-463.
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Released date1-Mar-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_000674.2
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Gene number152
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Protein numberP18825
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-51 (SMC-433). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (SMC-433) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Alpha 2C Adrenergic protein using Mouse Anti-Alpha 2C Adrenergic Monoclonal Antibody, Clone S330A-51 (SMC-433). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Alpha 2C Adrenergic Monoclonal Antibody (SMC-433) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Alpha-2C Adrenergic Receptor Antibody [S330A-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-433) Mouse Anti-Alpha 2C Adrenergic Antibody [S330A-51 ] used in Western Blot (WB) on Rat Brain Membrane (SMC-433)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThe Mouse Anti- Alpha 2C Adrenergic Monoclonal IgG2A, Clone S330A-51 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, ZNF3, SAMM50, OR7E26P
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Short nameMouse Anti- Alpha 2C Adrenergic Monoclonal IgG2A, Clone S330A-51
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2A, IgG2a
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LabelATTO 488
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman a 2C Adrenergic monoclonal IgG2A, clonality S330A-51
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Alternative techniquemurine, antibodies
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Clone nameS330A-51
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable IGHV8-51-1 (non-functional)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable (III)-51-1
- immunoglobulin heavy variable (III)-51-1 pseudogene
- immunoglobulin heavy variable (III)-51-1 (pseudogene)
- immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-51-2 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-51-2
- immunoglobulin heavy variable (II)-51-2 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-51
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-51
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 3
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Synonyms gene name
- zinc finger protein 3 (A8-51)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-31
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSAMM50 sorting and assembly machinery component
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Synonyms gene name
- sorting and assembly machinery component 50 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial sorting and assembly machinery complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 7 subfamily E member 26 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 7, subfamily E, member 26 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-29
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 7
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
