Mouse Anti-HA tag Monoclonal IgG3, Clone HA.C5
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Catalog numberSMC-260D-A594
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Price:
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Size100 µl
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CloneHA.C5
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ImmunogenA synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
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Antibody s full descriptionMouse Anti-Human HA tag Monoclonal IgG3 Antibody, Clone: HA.C5: ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody
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Raised inMouse
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Antibody s targetHA tag
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BrandnameNone
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Antibodies applicationsWB, ICC/IF, IP
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Antibody s reactivitySpecies Independent
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Antibody s dilutionsWB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storagePBS, pH 7.4, 50% glyercol, 0.05% sodium azide
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Antibody s specificityHA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 µg of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cellSee included datasheet or contact our support service
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Bibliography1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107–108.
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Released date06-Sep-2016
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red). Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000. Mouse Anti-HA Tag Antibody [HA.C5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on (SMC-260) Mouse Anti-HA Tag Antibody [HA.C5] used in Western Blot (WB) on Human Hek293 cell lysates (SMC-260)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti-HA tag Monoclonal IgG3, Clone HA C5
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, Tags
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Hostmouse
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IsotypeIgG3, IgG3
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LabelATTO 594
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toHA detection labelled monoclonal IgG3, clonality HA.complement component 5
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Alternative techniquemurine, antibodies
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Alternative to gene targetcomplement component 5, C5a and C5b and C5D and CPAMD4 and ECLZB, C5 and IDBG-83167 and ENSG00000106804 and 727, C5a anaphylatoxin chemotactic receptor binding, Extracellular, Hc and IDBG-163835 and ENSMUSG00000026874 and 15139, BT.24055 and IDBG-638834 and ENSBTAG00000012210 and 512045
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Clone nameHA
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data