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Mouse Anti-HA tag Monoclonal IgG3, Clone HA.C5

  • Catalog number
    SMC-260D-PCP
  • Product name
    Mouse Anti-HA tag Monoclonal IgG3, Clone HA.C5
  • Size
    100 µl
  • Label
    PerCP
  • Clone
    HA.C5
  • Immunogen
    A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
  • Antibody s full description
    Mouse Anti-Human HA tag Monoclonal IgG3 Antibody, Clone: HA.C5: PerCP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody
  • Raised in
    Mouse
  • Isotype
    IgG3, IgG3
  • Antibody s target
    HA tag
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, IP
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.
  • Antibody buffer for storage
    PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
  • Antibody s specificity
    HA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 µg of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Bibliography
    1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107–108.
  • Released date
    06-Sep-2016
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red). Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000. Mouse Anti-HA Tag Antibody [HA.C5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on (SMC-260) Mouse Anti-HA Tag Antibody [HA.C5] used in Western Blot (WB) on Human Hek293 cell lysates (SMC-260)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "complement component 5", "C5a and C5b and C5D and CPAMD4 and ECLZB", "C5 and IDBG-83167 and ENSG00000106804 and 727", "C5a anaphylatoxin chemotactic receptor binding", "Extracellular", "Hc and IDBG-163835 and ENSMUSG00000026874 and 15139", "BT.24055 and IDBG-638834 and ENSBTAG00000012210 and 512045" ]
  • Gene target
    HA tag Monoclonal IgG3 HA C5
  • Host
    mouse
  • isotype filter
    • IgG3
  • Short name
    Mouse Anti-HA tag Monoclonal IgG3, Clone HA C5
  • label filter
    • PerCP
  • Clone name
    HA
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, Tags
  • Alternative technique
    murine, antibodies