Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1, Clone KR-10
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Catalog numberSMC-129D-A655
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PricePlease ask
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Size100 µg
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CloneKR-10
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ImmunogenA 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
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Antibody s full descriptionMouse Anti-Bovine KDEL Receptor Monoclonal IgG1 Antibody, Clone: KR-10: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameERD2 Antibody, ERD2.1 Antibody, ERD21 Antibody, HDEL Antibody, KDEL Antibody, KDEL R1 Antibody, KDELR1 Antibody, PM23 Antibody
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Raised inMouse
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Antibody s targetKDEL Receptor
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Primary research fieldsCell Signaling, Organelle Markers, Trafficking, Chaperones
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP
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Antibody s reactivityHuman, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Pig, Sheep, Dog, Chicken, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
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Antibody s dilutionsWB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~25kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
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Antibody in cellEndoplasmic Reticulum
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
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Bibliography1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737. 2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219. 3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316. 4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328. 5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163. 6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from more
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Released date1-Nov-2008
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_598711.1
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Gene number68137
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Protein numberQ99JH8
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (SMC-129). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (SMC-129) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Courtesy of: Institute of Mol. and Cell Bio, Singapore. Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (SMC-129). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (SMC-129) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-KDEL Receptor Antibody [KR-10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat NRK cells (SMC-129) Mouse Anti-KDEL Receptor Antibody [KR-10] used in Western Blot (WB) on Rat tissue lysate (SMC-129)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolPIRC49, ZNF600, SNORD115-10, SNORD116-10, IGHV3OR16-10, IGKV2OR2-10, ERVK-10
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Short nameMouse Anti- KDEL Receptor Monoclonal IgG1, Clone KR-10
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 655
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SpeciesBovine, Bos Taurus genes are available form the Bovine Genome database. Mouses
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Alternative nameMouse Antibody toBovine KDEL Receptor monoclonal IgG1, clonality KR-10
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Alternative techniquemurine, antibodies
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Clone nameKR-10
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 49
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 600
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-10 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-10 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 10
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Synonyms gene name
- endogenous retrovirus group K, member 10
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Synonyms name
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GenBank acession
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Locus
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Discovery year2011-05-05
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Entrez gene record
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
