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Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1, Clone KR-10

  • Catalog number
    SMC-129D-A700
  • Product name
    Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1, Clone KR-10
  • Size
    100 µg
  • Label
    ATTO 700
  • Clone
    KR-10
  • Immunogen
    A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
  • Antibody s full description
    Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1 Antibody, Clone: KR-10: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ERD2 Antibody, ERD2.1 Antibody, ERD21 Antibody, HDEL Antibody, KDEL Antibody, KDEL R1 Antibody, KDELR1 Antibody, PM23 Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    KDEL Receptor
  • Primary research fields
    Cell Signaling, Organelle Markers, Trafficking, Chaperones
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Pig, Sheep, Dog, Chicken, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.2, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~25kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
  • Antibody in cell
    Endoplasmic Reticulum
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
  • Bibliography
    Bibliography
  • Released date
    1-Nov-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_598711.1
  • Gene number
    68137
  • Protein number
    Q99JH8
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (SMC-129). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (SMC-129) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Courtesy of: Institute of Mol. and Cell Bio, Singapore. Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (SMC-129). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (SMC-129) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-KDEL Receptor Antibody [KR-10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat NRK cells (SMC-129) Mouse Anti-KDEL Receptor Antibody [KR-10] used in Western Blot (WB) on Rat tissue lysate (SMC-129)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    - KDEL Receptor Monoclonal IgG1 KR-10
    • Gene info
    Gene info
    Gene info
    • Identity:HGNC:37553
    • Gene:PIRC49
    • Long gene name:piwi-interacting RNA cluster 49
    • Discovery year:2009-11-05
    • Entrez gene record:100313929
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    ZNF600, SNORD115-10, PIRC49, SNORD116-10, IGKV2OR2-10, IGHV3OR16-10, ERVK-10
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- KDEL Receptor Monoclonal IgG1, Clone KR-10
  • label filter
    • ATTO 700
  • Clone name
    KR-10
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies