MTHFR (A1298C) SNP-Screen RT-PCR test for detection of MTHFR gene mutation (Glu429Ala; Rs1801131), ready to use 0,2 ml tube format
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Catalog numberT01273-50-T
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Price:
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Size60 tests
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DescriptionThe detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
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GroupPCR, polymerase chain reaction, RT-PCR mixes
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbolMTHFR
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Short nameMTHFR (A1298C) SNP-Screen RT-PCR test for of MTHFR mutation (Glu429Ala; Rs1801131), 2 tube format
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Techniquedetection, RT-PCR, tube, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Sacace tubes
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Alternative nameMTHFR (A1298C) SNP-Screen Reverse transcription PCR test kit test to measure quantification on MTHFR gene mutation (Glu429Ala; Rs1801131), ready to use 0,2 milliliter tube supplied
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Alternative techniquertpcrkits, tests, tubes, dna-amplification
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Gene info
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Identity
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Gene
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Long gene namemethylenetetrahydrofolate reductase
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Synonyms gene name
- 5,10-methylenetetrahydrofolate reductase (NADPH)
- methylenetetrahydrofolate reductase (NAD(P)H)
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GenBank acession
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Locus
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Discovery year1994-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
- E05.393.620.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data