MTHFR (C677T) SNP-Screen RT-PCR test for detection of MTHFR gene mutation (Ala222Val; rs1801133), ready to use 12 x 8 strip format

• Catalog number
  T01103-96-S
• Price
  Please ask
• Size
  96 tests

• Description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva. 0,2 ml 8 well PCR test strip tubes and dip test strip are basic diagnostic tools used to determine pathological changes in a patient’s urine in standard urinalysis.
• Group
  PCR, polymerase chain reaction, RT-PCR mixes
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  MTHFR   C677T   SNP-Screen   test   for   MTHFR   gene   mutation   Ala222Val   rs1801133   format  
• Gene symbol
  MTHFR
• Short name
  MTHFR (C677T) SNP-Screen RT-PCR test for of MTHFR mutation (Ala222Val; rs1801133), 12 x 8 strip format
• Technique
  detection, RT-PCR, strip, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. strips
• Alternative name
  MTHFR (C677T) SNP-Screen Reverse transcription PCR test kit test to measure quantification on MTHFR gene mutation (Ala222Val; rs1801133), ready to use 12 x 8 strip supplied
• Alternative technique
  rtpcrkits, strips, tests, dna-amplification

Gene info

• Identity
  HGNC:7436
• Gene
  MTHFR
• Long gene name
  methylenetetrahydrofolate reductase
• Synonyms gene name
  • 5,10-methylenetetrahydrofolate reductase (NADPH)
  • methylenetetrahydrofolate reductase (NAD(P)H)
• GenBank acession
  • BC053509
• Locus
  1p36.22
• Discovery year
  1994-07-15
• Entrez gene record
  4524
• Pubmed identfication
  • 7920641
• RefSeq identity
  • NM_005957
• VEGA ID
  OTTHUMG00000002277
• Locus Specific Databases
  • LRG_726

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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