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Gene names
FPF, MS5, p55, p60, TBP1, TNF-R, TNFAR, TNFR1, p55-R, CD120a, TNFR55, TNFR60, TNF-R-I, TNF-R55, TNFR1-d2, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1
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Antibodie s label
unlabelled serum
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Purity
Anti-hsTNF Receptor-I specific antibody was purified by affinity chromatography employing immobilized hsTNF Receptor-I matrix.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF Receptor-I-I (human-soluble Tumor Necrosis Factor Receptor-I).
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsTNF Receptor-I (0.3 ug/mL), a concentration of 0.9-1.1 ug/mL of this antibody is required. ELISA:To detect hsTNF Receptor-I by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsTNF Receptor-I. Sandwich:To detect hsTNF-receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sTNF-receptor (XP-5276Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.Western Blot:To detect hsTNF Receptor-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF Receptor-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
sTNF Receptor Type I antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
135959
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Ncbi nr
P19438
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Vial with antibody
Lyophilized
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NCBI GENE nr
7132
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GENE symbol
TNFRSF1A
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Antigene name
tumor necrosis factor receptor superfamily, member 1A
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Source
Homo sapiens
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Protein nr
P19438
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps