anti-SMAD4 Antibody

• Catalog number
  27-474
• Price
  Please ask
• Size
  100 ug

• Gene info
  SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
• Gene names
  SMAD4, JIP, DPC4, MADH4, MYHRS
• Antibodie s label
  unlabelled serum
• Purity
  Antibody is purified by protein A chromatography method.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.
• Immuno application
  ELISA, Western Blot
• Antibody advice
  SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Suspention pH azide
  Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
• Keep at
  For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD4 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
• Works with
  Human
• Omim nr
  4885457
• Ncbi nr
  NP_005350
• KDa
  60 kDa
• Vial with antibody
  Lyophilized
• Quantity per ml
  1 mg/ml
• NCBI GENE nr
  4089
• GENE symbol
  SMAD4
• Antigene name
  SMAD family member 4
• Source
  Homo sapiens
• Protein nr
  Q13485
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SMAD4  
• Gene symbol
  SMAD4
• Short name
  anti-SMAD4 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toSMAD4 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  SMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248

Gene info

• Identity
  HGNC:6770
• Gene
  SMAD4
• Long gene name
  SMAD family member 4
• Synonyms gene
  • MADH4
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4 (Drosophila)
• Synonyms
  • DPC4
• GenBank acession
  • U44378
• Locus
  18q21.2
• Discovery year
  1996-11-15
• Entrez gene record
  4089
• Pubmed identfication
  • 8553070
  • 8774881
• RefSeq identity
  • NM_005359
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000132696
• Locus Specific Databases
  • LRG_318

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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