ImProm-II Reverse Transcriptase

Ambiant Carboglace

Dry or Gel Ice
Description

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup

reverse transcription

Gene target

ImProm-II Transcriptase
Gene symbol

SPINK2, CRABP2, MGST2, IBSP, APOL2, MGAT2, P4HA2, XYLT2, TMED10P1, PSMB3
Short name

ImProm-II Reverse Transcriptase
Technique

reverse
Alternative name

ImProm-II Reverse Transcriptase
Alternative technique

transctription

Identity

HGNC:11245
Gene

SPINK2
Long gene name

serine peptidase inhibitor Kazal type 2
Synonyms gene name
- serine protease inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
Synonyms
- HUSI-II
Synonyms name
- acrosin-trypsin inhibitor
GenBank acession
- BC022514
Locus

4q12
Discovery year

1993-12-01
Entrez gene record

6691
Pubmed identfication
- 8428671
RefSeq identity
- NM_021114
Classification
- Serine peptidase inhibitors, Kazal type
VEGA ID

OTTHUMG00000128769

Identity

HGNC:7045
Gene

MGAT2
Long gene name

alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synonyms gene name
- mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Synonyms
- GNT-II
GenBank acession
- U15128
Locus

14q21.3
Discovery year

1993-02-16
Entrez gene record

4247
Pubmed identfication
- 7635144
RefSeq identity
- NM_002408
Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
VEGA ID

OTTHUMG00000140271
Locus Specific Databases
- Congenital Disorder of Glycosylation pages

Identity

HGNC:8547
Gene

P4HA2
Long gene name

prolyl 4-hydroxylase subunit alpha 2
Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
- prolyl 4-hydroxylase, alpha polypeptide II
Synonyms
- C-P4Halpha(II)
Synonyms name
- 4-PH alpha 2
- collagen prolyl 4-hydroxylase alpha(II)
GenBank acession
- U90441
Locus

5q31.1
Discovery year

1999-02-23
Entrez gene record

8974
Pubmed identfication
- 9211872
- 9149945
RefSeq identity
- NM_004199
Classification
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000059647

Identity

HGNC:30754
Gene

TMED10P1
Long gene name

transmembrane p24 trafficking protein 10 pseudogene 1
Synonyms gene
- TMED10P
Synonyms gene name
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene 1
Synonyms
- Tmp21-II
GenBank acession
- AJ004914
Locus

8q24.3
Discovery year

2005-08-26
Entrez gene record

286102
Pubmed identfication
- 10376215
RefSeq identity
- XR_002807
VEGA ID

OTTHUMG00000165203

Identity

HGNC:9540
Gene

PSMB3
Long gene name

proteasome 20S subunit beta 3
Synonyms gene name
- proteasome (prosome, macropain) subunit, beta type, 3
- proteasome subunit beta 3
Synonyms
- HC10-II
- MGC4147
GenBank acession
- BC013008
Locus

17q12
Discovery year

1995-05-03
Entrez gene record

5691
Pubmed identfication
- 7918633
RefSeq identity
- NM_002795
Classification
- Proteasome
VEGA ID

OTTHUMG00000188503

Name

Reverse Transcriptase Polymerase Chain Reaction
Concept

Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tree numbers
- E05.393.620.500.725
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data