SCRIPT Reverse Transcriptase
-
Catalog number
PCR-505S
-
Price
Please ask
-
Size
500units
-
-
Shipping Conditions
shipped on blue ice
-
Storage Conditions
store at -20 °C.avoid freeze/thaw cycles.
-
Shelf Life
12 months
-
-
-
UNSPSC Code
41106300
-
UNSPSC Code Description
Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
-
Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
-
Goup
reverse transcription
MeSH Data
-
Name
-
Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
-
Tree numbers
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products