NAD-dependent DNA Ligase Assay Kit (enzyme not included)

Full description

NAD-dependent DNA Ligase Assay Kit (enzyme not included) includes 800 µl of 10 x buffer, 44 µl of 100x DNA substrate, 44 µl of 100 x NAD+, 21 ml of Reagent T and 220 µl of 10 x fluorescence dye for 100 assays. Reagent T is used to stop the reaction and denature the DNA at the end of the ligase reaction. The kit does not include ligase that can be purchased separately (please see the information of related products).
Partial description

Reagents for fluorescence-based 384-well or 96-well assays of NAD-dependent DNA ligase, 100 assays, enzyme is not included
Product type

Bacterial DNA ligases and assays
Shipped to you

This product is shipped to you frozen
Ordering

Product made-to-order. Delivery is 1-2 weeks upon order confirmation. Please contact us to check the current inventory stock. For larger or bulk quantity a discount may apply.
Precautions

For Research Use Only. Produced in USA
Other products
Test

ProF supplies other types of Assays as 1.
Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Gene target

NAD-dependent DNA Ligase Kit not included
Gene symbol

PRKDC, HLA-DOA
Short name

NAD-dependent DNA Ligase Assay Kit (enzyme not included)
Technique

Enzyme, Assay, dna, assays, enzymes
Host

Assay
Alternative name

Nicotinamide adenine dinucleotide-dependent Desoxyribonucleic acid Ligase test reagent (enzyme not included)
Alternative technique

enzymes, arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data