Recombinant ATP Dependent DNA ligase I (LIG1)

Verified reactivity

Homo sapiens (Human)
Protein number

P18858
Gene number

Please refer to GenBank
Other name

Polydeoxyribonucleotide synthase [ATP] 1
Protein origin

E.coli
Protein region

Please contact us.
Protein sequence

Please contact us.
Information about sequence

Gly484~Ile663
Expected molecular weight

24.4kDa
Protein purity

≥ 95%
Verified applications

SDS-PAGE; WB; ELISA; IP.
Storage recommendation

Aliquot and store at -20°C. Minimize freezing and thawing.
Use before

1 year
Shipping requirements

Blue ice
Estimated production time

7-11 business days
Notes

For research use only. Not for diagnostic procedures.
Hyperlink
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

ATP Dependent DNA ligase LIG1
Gene symbol

LIG1, VPS9D1, LRIG1, PRKDC, NFATC2, HLA-DOA
Short name

Recombinant ATP Dependent DNA ligase I (LIG1)
Technique

Recombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. bioma advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Label

two N-terminal Tags, His-tag and T7-tag
Alternative name

Rec. adenosine triphosphate Dependent Desoxyribonucleic acid ligase I (LIG1)
Alternative technique

rec

Identity

HGNC:6598
Gene

LIG1
Long gene name

DNA ligase 1
Synonyms gene name
- ligase I, DNA, ATP-dependent
Synonyms name
- DNA ligase I
Locus

19q13.33
Discovery year

1991-05-09
Entrez gene record

3978
Pubmed identfication
- 1733856
RefSeq identity
- NM_000234
Classification
- Nucleotide excision repair
- DNA ligases
VEGA ID

OTTHUMG00000183187
Locus Specific Databases
- LIG1base: Mutation registry for DNA ligase I deficiency
- LRG_78

Identity

HGNC:17360
Gene

LRIG1
Long gene name

leucine rich repeats and immunoglobulin like domains 1
Synonyms
- LIG-1
- DKFZP586O1624
- LIG1
Synonyms name
- ortholog of mouse integral membrane glycoprotein LIG-1
- leucine-rich repeat protein LRIG1
GenBank acession
- AB050468
Locus

3p14.1
Discovery year

2002-08-23
Entrez gene record

26018
Pubmed identfication
- 11414704
- 12234026
RefSeq identity
- NM_015541
Classification
- I-set domain containing
VEGA ID

OTTHUMG00000158727

Identity

HGNC:7776
Gene

NFATC2
Long gene name

nuclear factor of activated T cells 2
Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
Synonyms
- NF-ATP
- NFATp
- NFAT1
GenBank acession
- U43342
- U43341
Locus

20q13.2
Discovery year

1994-11-16
Entrez gene record

4773
Pubmed identfication
- 8202141
RefSeq identity
- NM_012340
Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000032747

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data