E. coli Biotin Protein Ligase Assay Kit Plus

Full description

E. coli Biotin Protein Ligase Assay Kit Plus contains the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x Biotin, 0.035 ml of 100 x hydroxylamine, 0.035 ml of 100 x ATP, 0.035 ml of 100 x E. coli BirA (5000 nM), 0.035 ml of 100 x pyrophosphatase (PPase, 10 U/ml) and 5 ml of Dye for phosphate detection.
Partial description

Reagents for fluorescence-based 384-well or 96-well assays of E. coli Biotin Protein Ligase activity, 100 assays
Product type

other antibacterial drug targets
Shipped to you

This product is shipped to you frozen
Ordering

Product made-to-order. Delivery is 1-2 weeks upon order confirmation. Please contact us to check the current inventory stock. For larger or bulk quantity a discount may apply.
Precautions

For Research Use Only. Produced in USA
Other products
Test

ProF supplies other types of Assays as 1.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Gene target

Protein Ligase Kit Plus
Short name

Protein Ligase Assay Kit Plus
Technique

Assay, assays
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. ProF supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Label

Biotin
Species

E. coli, E. coli
Alternative name

E. coli biotinilated Protein Ligase test reagent Plus
Alternative technique

arrays, escherichia, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data