General Block ELISA Blocking Buffer

CAT: 0246-640Size: 1 LDry Ice: NoHazardous: No
CAT#:0246-640Size:1 L
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Description
Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.
CAS Number
7732-18-5
Assay Principle
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer., 2. Incubate 8–24 hours at room temperature (RT)., 3. Aspirate the coating solution., 4. Wash each well twice with ICT’s ELISA Wash Buffer., 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution., 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT., 7. Aspirate the blocking buffer; do not wash., 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.
Concentration
1X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture

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