Sheep anti Human Cytochrome c

Sheep anti Human Cytochrome c

• Background: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain._x000B_Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases._x000B_Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
• Synonyms: Sheep Anti cytochrome c, cyto c, cytochrome-c
• CAS Number: 9007-83-4
• UniProt: P99999 (Human), P62898 (Rat), P00008 (Rabbit), P00011 (Dog)
• Host: Sheep
• Species Reactivity: Human, Rat, Rabbit, Dog
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Applications: WB, IP, IHC
• Field of Research: Apoptosis
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Applications: Western Blot 1:5,000, shows a MW of 14.4kDa. May be used in immunofluorescence applications with Human and rat cells; Immunoprecipitation - Empirical.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Morison, I.M., et al. 'A mutation of Human cytochrome c enhances the intrinsic apoptotic pathway but causes only thrombocytopenia.' Nat. Genet. 2008, 40, 387-389 2. Skulachev, V.P. 'Cytochrome c is the apoptotic and antioxidant cascades.’ FEBS Lett 1998, 423, 275-280
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB