E2F-2 (Acetyl Lys122) rabbit pAb

E2F-2 (Acetyl Lys122) rabbit pAb

• Description: Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner. PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. similarity: Belongs to the E2F/DP family. subunit: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP. tissue specificity: Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
• Synonyms: Transcription factor E2F2 (E2F-2)
• Gene ID: 1870
• UniProt: Q14209
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthesized peptide derived from human E2F-2 (Acetyl Lys122)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1000-2000 ELISA 1:5000-20000
• Molecular Weight: 48kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.