Anti-PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF647 conjugate

• Description: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SPI1
• Gene ID: 6688
• NCBI Gene ID: 502511
• UniProt: P17947
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
• Target Antigen: PU.1
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: PU1/2146
• Conjugation: CF647
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Immunology, Transcription factors
• Positive Control: K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 40 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Western Blot 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years