Anti-Interleukin 10 (IL10) (Immunoregulation Marker) (IL10/2651R), CF647 conjugate

• Description:

  Interleukin 10 (IL10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immuno-regulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immuno-regulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cytokine Synthesis Inhibitory Factor (CSIF); GVHDS; IL-10; IL10; IL10A; Interleukin-10; T-cell Growth Inhibitory Factor (TGIF)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  IL10

• Gene ID:

  3586

• NCBI Gene ID:

  193717

• UniProt:

  P22301

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human Interleukin 10 (IL10) protein

• Target Antigen:

  Interleukin 10

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  IL10/2651R

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cytokines, Immunology, Inflammation

• Positive Control:

  SK-BR3 Cells. Adrenal, Lymph Node, Bladder, Prostate or Endometrium.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  20 kDa (Monomer); 37 kDa (Dimer)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4