Anti-HLA-DQ (MHC II)(HLA-DQA1/2866R), CF740 conjugate

• Description: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CELIAC1; DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE); HLA class II histocompatibility antigen DQ(1) alpha chain; HLA class II histocompatibility antigen DQ(3) alpha chain; HLA DQA1; HLA DQB1; IDDM1; Leucocyte antigen DQA1; Major histocompatibility complex class II DQ alpha 1; Major histocompatibility complex class II DQ beta 1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HLA-DQ
• Gene ID: 3117; 3118; 3119
• NCBI Gene ID: 550475
• UniProt: P01908; P01909; P01920
• Cellular Locus: Plasma membrane
• Host: Rabbit
• Species Reactivity: Human, Pig
• Immunogen: Recombinant full-length human HLA-DQ protein
• Target Antigen: HLA-DQ
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: HLA-DQA1/2866R
• Conjugation: CF740
• Source: Animal
• Applications: Flow (verified) | IF (verified)
• Validated Applications: FC, IF
• Positive Control: Raji cells. Tonsil or lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 60 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years