Anti-TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker)(TIM3/3113), CF740 conjugate

Anti-TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker)(TIM3/3113), CF740 conjugate

• Description: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4 Th1 and CD8 Tc1) but not on helper T cells (CD4 Th2 and CD8 Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD366; HAVR2; Hepatitis A virus cellular receptor 2 (HAVCR2); Kidney injury molecule 3 (KIM3); T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD3
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HAVCR2
• Gene ID: 84868
• NCBI Gene ID: 710500
• UniProt: Q8TDQ0
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)
• Target Antigen: CD366 | HAVCR2 | TIM3
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: TIM3/3113
• Conjugation: CF740
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Positive Control: PC3, BT474, HepG2, HDLM-2 or Daudi cells. Tonsil, lymph node or spleen.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 60 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years