Anti-CD5 (Mantle Cell Lymphoma Marker) (CD5/2416), Biotin conjugate
CAT:
37-BNCB2416-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-CD5 (Mantle Cell Lymphoma Marker) (CD5/2416), Biotin conjugate
- Description: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma ( ) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: CD5
- Gene ID: 921
- NCBI Gene ID: 58685
- UniProt: P06127
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)
- Target Antigen: CD5
- Clonality: Monoclonal
- Isotype: IgG2b κ
- Clone: CD5/2416
- Conjugation: Biotin
- Disease: Tumor
- Source: Animal
- Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
- Validated Applications: FC, IF, IHC, FFPE, WB
- Field of Research: Immunology
- Positive Control: 293T, Ramos, or MOLT-4 Cells. Tonsil or Lymph Node
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% BSA, 0.05% azide
- Molecular Weight: 67 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Western Blot 0.5-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years