Anti-GAD2 / GAD65 (GABAergic Neuronal Marker) (GAD2/1960), Biotin conjugate

• Description:

  This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (ÃŽ³-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_

• Synonyms:

  DCE2; GAD2; Glutamic Acid Decarboxylase 2 (Pancreas); Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65kDa); Glutamic Acid Decarboxylase 65 (GAD65)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  GAD2

• Gene ID:

  2572

• NCBI Gene ID:

  231829

• UniProt:

  Q05329

• Cellular Locus:

  Cytoplasmic|Plasma membrane|Vesicular

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

• Target Antigen:

  GAD2 | GAD65

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  GAD2/1960

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Neuroscience

• Positive Control:

  Pancreas or Brain (IHC).

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  65 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4