Anti-PAX5 / BSAP (Early B-Cell Marker) (PCRP-PAX5-1B7)

Anti-PAX5 / BSAP (Early B-Cell Marker) (PCRP-PAX5-1B7)

• Description: The specificity of this monoclonal antibody to its intended target was validated by HuProt™ Array, containing more than 19,000, full-length human proteins. The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: B cell lineage specific activator; BSAP; EBB-1; KLP; Paired box 5
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PAX5
• Gene ID: 5079
• NCBI Gene ID: 654464
• UniProt: Q02548
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human PAX5 protein
• Target Antigen: BSAP | PAX5
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: PCRP-PAX5-1B7
• Conjugation: Purified, with BSA
• Source: Animal
• Field of Research: Developmental biology, Immunology, Transcription factors
• Positive Control: Raji, DAUDI or Ramos Cells. Tonsil, spleen, Lymph Node or Colon Carcinoma.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 42 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Desouki MM., et al. Clin Med Res. 2010 Jul;8(2):84-8.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years