Anti-Cytokeratin 7 (Glandular and Transitional Epithelial Marker) (rOV-TL12/30) | Gentaur

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Anti-Cytokeratin 7 (Glandular and Transitional Epithelial Marker) (rOV-TL12/30)

CAT:

37-BNUM1841-50

Size:

50 µL

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Anti-Cytokeratin 7 (Glandular and Transitional Epithelial Marker) (rOV-TL12/30)

Description: This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Synonyms: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT7
Gene ID: 3855
NCBI Gene ID: 411501
UniProt: P08729
Cellular Locus: Cytoskeleton
Host: Mouse
Species Reactivity: Human
Immunogen: OTN 11|ovarian carcinoma cell line
Target Antigen: Cytokeratin 7
Clonality: Recombinant Monoclonal
Isotype: IgG1
Clone: rOV-TL12/30
Conjugation: Purified, BSA-free
Disease: Tumor
Source: Animal
Applications: Flow, intracellular (verified) | IHC, FFPE (verified) | WB (verified)
Validated Applications: FC, IHC, FFPE, WB
Field of Research: Cancer, Cytoskeleton
Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix, or breast
Concentration: 1 mg/mL
Buffer: PBS, no BSA, no azide
Molecular Weight: 55 kDa
Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Shipping Conditions: Room temperature
Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: 2 years

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