Anti-CD34 (Hematopoietic Stem Cell & Endothelial Marker) (HPCA1/1806R)

Anti-CD34 (Hematopoietic Stem Cell & Endothelial Marker) (HPCA1/1806R)

• Description: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD34
• Gene ID: 947
• NCBI Gene ID: 374990
• UniProt: P28906
• Cellular Locus: Plasma membrane
• Host: Rabbit
• Species Reactivity: Human, Rat
• Immunogen: Recombinant full-length human HPCA1 protein
• Target Antigen: CD34
• Clonality: Recombinant Monoclonal
• Isotype: IgG κ
• Clone: HPCA1/1806R
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IHC, FFPE
• Field of Research: Angiogenesis, Cancer
• Positive Control: KG-1 cells, Tonsil, or Angiosarcoma
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 90-110 kDa
• Additionnal Information: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Immunohistology (formalin) 2-4 ug/mL|Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years