Anti-Calnexin (Endoplasmic Reticulum Marker) (CANX/1541)

Anti-Calnexin (Endoplasmic Reticulum Marker) (CANX/1541)

• Description: Calnexin is a type-I integral membrane protein of the endoplasmic reticulum (ER). It is a calcium-binding chaperone protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation._x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Calnexin; CANX; IP90; Major histocompatibility complex class I antigen-binding protein p88
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CANX
• Gene ID: 821
• NCBI Gene ID: 567968
• UniProt: P27824
• Cellular Locus: Endoplasmic reticulum
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)
• Target Antigen: Calnexin
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: CANX/1541
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow, intracellular | IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Chaperones, Immunology
• Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 67 kDa (predicted); 80-90 kDa (observed)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years