Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) (P-GF.44C)

Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) (P-GF.44C)

• Description: This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ECGF; ECGF1; Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PD-ECGF; PDECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; Tymp
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TYMP
• Gene ID: 1890
• NCBI Gene ID: 180903
• UniProt: P19971
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein
• Target Antigen: PD-ECGF | Thymidine Phosphorylase
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: P-GF.44C
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Angiogenesis, Metabolism
• Positive Control: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 55 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin) 1-2 ug/mL|Western blotting 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500ug protein lysate|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years