Thymidine Phosphorylase Recombinant Antibody / PD-ECGF

Thymidine Phosphorylase Recombinant Antibody / PD-ECGF

• Description: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
• CAS Number: 9000-83-3
• UniProt: P19971
• Host: Mouse
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant PD-ECGF antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Type: Recombinant
• Applications: IHC-P, WB
• Format: Purified
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Reconstitution: Store the recombinant PD-ECGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This recombinant PD-ECGF antibody is available for research use only.