Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) (MAML2/1302), Biotin conjugate

Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) (MAML2/1302), Biotin conjugate

• Description: MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Mam-2; MAML2; Mastermind-like protein 2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: MAML2
• Gene ID: 84441 (Human)
• NCBI Gene ID: 428214 (Human)
• UniProt: Q8IZL2 (Human)
• Cellular Locus: Cytoplasmic|Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human full-length MAML2
• Target Antigen: MAML2
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: MAML2/1302
• Conjugation: Biotin
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Developmental biology, Signal transduction
• Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 125 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years