Anti-ALDH1A1 (Aldehyde Dehydrogenase 1A1) (ALDH1A1/1381), CF568 conjugate

• Description:

  Aldehyde dehydrogenase 1 family member A1 (ALDH1A1), also known as retinal dehydrogenase 1, belongs to the aldehyde dehydrogenase enzyme family, which is involved in the metabolism of alcohol. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Aldehyde dehydrogenase family 1 member A1; ALDH1A1; Retinal dehydrogenase 1; Retinaldehyde Dehydrogenase 1; Epididymis Luminal Protein

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ALDH1A1

• Gene ID:

  216

• NCBI Gene ID:

  76392

• UniProt:

  P00352

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Purified recombinant fragment of human ALDH1A1 (aa 315-434) (exact sequence is proprietary)

• Target Antigen:

  Aldehyde dehydrogenase 1

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  ALDH1A1/1381

• Conjugation:

  CF568

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Metabolism

• Positive Control:

  HepG2 or A549 cells. Colon or Testicular Carcinoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  56 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4