SCRIPT Reverse Transcriptase

• Description:

  SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.

• Product Name Alternative:

  Reverse Transcriptase with increased thermal stability

• Synonyms:

  Reverse Transcriptase with increased thermal stability

• UNSPSC:

  41106300

• UNSPSC Description:

  Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products

• HS Code:

  35079090

• eClass Number:

  32160502

• eClass Product Group:

  PCR-Reagenz (Labor)

• Applications:

  Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.

• Concentration:

  200 units/μL

• Purity:

  free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease

• Form:

  Liquid

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Avoid freeze/thaw cycles

• Storage Temperature:

  Store at -20 °C

• Shelf Life:

  12 months