SCRIPT Reverse Transcriptase

• Description :

  SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.

• Product Name Alternative :

  Reverse Transcriptase with increased thermal stability

• Synonyms :

  Reverse Transcriptase with increased thermal stability

• UNSPSC :

  41106300

• UNSPSC Description :

  Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products

• HS Code :

  35079090

• eClass Number :

  32160502

• eClass Product Group :

  PCR-Reagenz (Labor)

• Applications :

  Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.

• Concentration :

  200 units/μL

• Purity :

  free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease

• Form :

  Liquid

• Autoclavability :

  false

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Avoid freeze/thaw cycles

• Storage Temperature :

  Store at -20 °C

• Shelf Life :

  12 months