Thermolabile UNG (Uracil N-Glycosylase) - 10 u/µl UNG (UDG) for preventing carry-over contaminations in PCR assays

Thermolabile UNG (Uracil N-Glycosylase) - 10 u/µl UNG (UDG) for preventing carry-over contaminations in PCR assays

• Description: Thermolabile UNG (UDG) is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. The enzyme is fully active at temperatures up to 55°C and inactivated above 65°C. Thermolabile Uracyl N-Glycosylase (UNG, UDG) catalyzes the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.
• UNSPSC: 41106300
• UNSPSC Description: Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
• HS Code: 35079090
• eClass Number: 32160502
• eClass Product Group: PCR Reagent (Laboratory)
• Concentration: 10 unit/μL
• Form: Liquid
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months