Thermolabile UNG (Uracil N-Glycosylase) Prevention of carry-over contaminations

Thermolabile UNG (Uracil N-Glycosylase) Prevention of carry-over contaminations

• Description: Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.
• UNSPSC: 41106300
• UNSPSC Description: Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
• HS Code: 35079090
• eClass Number: 32160502
• eClass Product Group: PCR Reagent (Laboratory)
• Concentration: 1 unit/μL
• Form: Liquid (Supplied in 20 mM Tris-HCl pH 8.0, 50 mM NaCl,1 mM EDTA, 1 mM DTT, 100 μg/ml BSA and 50 % [v/v] glycerol)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months