RNase I (DNase free) Endonuclease recombinant, E. coli

• Description:

  Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.

• CAS Number:

  9000-83-3

• UNSPSC:

  12352204

• UNSPSC Description:

  Enzymes

• HS Code:

  35079090

• eClass Number:

  34160500

• eClass Product Group:

  Enzyme and Coenzyme

• Applications:

  degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate Ribonuclease Protection Assays Mapping or Quantitation of RNA by selective cleavage of single-strand regions Eliminates RNA from DNA- and protein purification

• Purity:

  > 95 % (SDS-PAGE)

• Form:

  Liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol)

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs.

• Storage Conditions:

  Store at -20 °C. Avoid freeze/thaw cycles.

• Shelf Life:

  12 months