RNase I (DNase free) Endonuclease recombinant, E. coli

RNase I (DNase free) Endonuclease recombinant, E. coli

• Description: Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.
• CAS Number: 9000-83-3
• UNSPSC: 12352204
• UNSPSC Description: Enzymes
• HS Code: 35079090
• eClass Number: 34160500
• eClass Product Group: Enzyme and Coenzyme
• Applications: degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate Ribonuclease Protection Assays Mapping or Quantitation of RNA by selective cleavage of single-strand regions Eliminates RNA from DNA- and protein purification
• Purity: > 95 % (SDS-PAGE)
• Form: Liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months