RNase I (DNase free) Endonuclease recombinant, E. coli
CAT:
220-EN-176L
Size:
5 x 2000 Units
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

RNase I (DNase free) Endonuclease recombinant, E. coli
- Description: Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.
- CAS Number: 9000-83-3
- UNSPSC: 12352204
- UNSPSC Description: Enzymes
- HS Code: 35079090
- eClass Number: 34160500
- eClass Product Group: Enzyme and Coenzyme
- Applications: degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate Ribonuclease Protection Assays Mapping or Quantitation of RNA by selective cleavage of single-strand regions Eliminates RNA from DNA- and protein purification
- Purity: > 95 % (SDS-PAGE)
- Form: Liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol)
- Autoclavability: false
- Shipping Conditions: Shipped on gel packs.
- Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
- Shelf Life: 12 months