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C8-Alkyne-dCTP

CAT: 0220-CLK-T06-SSize: 10 µLDry Ice: NoHazardous: No
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CAT#:0220-CLK-T06-SSize:10 µL
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UNSPSC
12352200
UNSPSC Description
Biochemicals
HS Code
29349990
eClass Number
32160405
eClass Product Group
Biochemikalie (Labor, Sonstige)
Applications
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1] Incorporation into DNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase(TdT)[3] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Concentration
(100 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
Smiles
C1(nc(=O)n(cc1C#CCCCCC#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)N
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
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Product MSDS
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