C8-Alkyne-dUTP

• UNSPSC:

  12352200

• UNSPSC Description:

  Biochemicals

• HS Code:

  29349990

• eClass Number:

  32160405

• eClass Product Group:

  Biochemikalie (Labor, Sonstige)

• Applications:

  Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

• Concentration:

  (100 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Solution in water

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  C1 (=O) [nH]c (=O) n (cc1C#CCCCCC#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery