Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]

• Reactivity:

  Goat

• Immunogen:

  Goat IgG, whole molecule

• Type:

  Antibodies-Secondary

• Applications:

  Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase- based enzymatic assays.

• Concentration:

  10.0 mg/ml (by UV absorbance at 280 nm)

• Purity:

  This product is an IgG fraction purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.

• Buffer:

  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

• Applications Notes:

  This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

• CAS Number:

  9007-83-4