Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat]

• Description:

  Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins

• Reactivity:

  Rabbit

• Immunogen:

  Rabbit IgG, whole molecule

• Type:

  Antibodies-Secondary

• Applications:

  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.

• Concentration:

  1.0 mg/ml (by UV absorbance at 280 nm)

• Purity:

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

• Buffer:

  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

• Applications Notes:

  FLOW CYTOMETRY 1:200 - 1:400 IF MICROSCOPY 1:100 - 1:1,000 OTHER APPLICATIONS User Optimized Pre-Conjugation ELISA Data Minimum ELISA Endpoint Dilution: 1:10,000 Actual: 1:41,000 Disposition: PASS

• CAS Number:

  9007-83-4