Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

• Reactivity:

  Human

• Immunogen:

  Human IgG, whole molecule

• Type:

  Antibodies-Secondary

• Applications:

  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.

• Concentration:

  1.0 mg/ml (by UV absorbance at 280 nm)

• Purity:

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

• Buffer:

  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

• Applications Notes:

  Not specified. Optimal titers for applications should be determined by the researcher.

• CAS Number:

  9007-83-4