Anti-DFFA

• Description:

  Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide conjugated to KLH

• Clonality:

  Polyclonal

• Type:

  Antibodies-Polyclonal

• Applications:

  WB,ELISA

• Concentration:

  100ug/100ul

• Purity:

  Protein A purified

• Buffer:

  PBS, pH 7.4 with 0.02% Sodium Azide

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Fragment:

  Rabbit IgG

• Specificity:

  DFFA antibody detects endogenous levels of DFFA protein. Not tested with other proteins

• Applications Notes:

  Recommended dilutions Peptide ELISA: 1:40,000 WB: 1/200 - 1/1000 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user

• CAS Number:

  9007-83-4