Anti-FLI1 (Ewing s Sarcoma & Endothelial Marker) (FLI1/1312), CF647 conjugate

• Description:

  This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ERGB transcription factor; Ewing Sarcoma breakpoint region 2 (EWSR2); FLI1; FLI1 EWS fusion gene; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC1; Transcription factor ERGB; Viral integration region FLI1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FLI1

• Gene ID:

  2313

• NCBI Gene ID:

  504281

• UniProt:

  Q01543

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)

• Target Antigen:

  FLI1

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  FLI1/1312

• Conjugation:

  CF647

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  WB (verified)

• Validated Applications:

  WB

• Field of Research:

  Cancer, Immunology, Transcription factors

• Positive Control:

  THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  51 kDa

• Additionnal Information:

  Western blotting 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years