Recombinant Rat IL1B

• Description:

  Monocytes are the main source of secreted IL-1. They express predominantly IL-1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B cells, granulocytes, and macrophages. It is expressed predominantly on B cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

• Gene Name:

  IL1B

• Gene Aliases:

  IL-1b
  Recombinant Rat Interleukin-1 Beta (IL1B)

• Gene ID:

  24494

• Accession Number:

  Q63264

• Organism:

  Rat

• Source:

  E. coli

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/mL, corresponding to a specific activity of ≥ 5.0 x 10^8 units/mg.

• Molecular Weight:

  17

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Rat IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

• CAS Number:

  9000-83-3