Recombinant Mouse IL1B

• Description :

  Monocytes are the main source of secreted IL-1. They express predominantly IL1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

• Gene Name :

  IL1B

• Gene Aliases :

  IL-1 beta, IL1b
  Recombinant Mouse Interleukin-1 Beta (IL1B)

• Gene ID :

  16176

• Accession Number :

  P10749

• Organism :

  Mouse

• Source :

  E. coli

• Concentration :

  < 1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity :

  >95% as determined by SDS-PAGE

• Solubility :

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function :

  The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/mL, corresponding to a specific activity of ≥ 5.0 x 10^8 units/mg.

• Molecular Weight :

  17

• Shipping Conditions :

  Ambient Temperature

• Storage Conditions :

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance :

  Lyophilized Powder

• Formulation :

  Mouse IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

• CAS Number :

  9000-83-3