Recombinant Rat Eotaxin (CCL11)

• Description:

  Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. This factor is referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.

• CAS Number:

  9000-83-3

• Gene Name:

  CCL11

• Gene Aliases:

  C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, Scya11

• Gene ID:

  29397

• Accession Number:

  P97545

• Organism:

  Rat

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  Activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of 0.1-20 ng/mL.

• Molecular Weight:

  8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS