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Recombinant Mouse MIF

CAT: 0171-Z200295Size: 25 µgDry Ice: NoHazardous: No
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CAT#:0171-Z200295Size:25 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macrophage Migration Inhibitory Factor (MIF) is a proinflammatory cytokine which is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
CAS Number
9000-83-3
Gene Name
MIF
Gene Aliases
MIF, EC=5.3.2.1, Delayed early response protein 6, DER6, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, EC=5.3.3.12, Phenylpyruvate tautomerase
Recombinant Mouse Macrophage Migration Inhibitory Factor (MIF)
Gene ID
17319
Accession Number
P34884
Organism
Mouse
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED(50) was determined by the dose-dependent proliferation of HTB9 cells and was found to be <0.5ng/mL.
Molecular Weight
15
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Recombinant mouse MIF was lyophilized from a 0.2 μm filtered solution PBS pH 7.4.

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